Genotype differentiating RT-PCR and sandwich ELISA: Handy tools in epidemiological investigation of foot and mouth disease

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• 作者：Jajati Keshari Mohapatra ; Saravanan Subramaniam ; Chakradhar Tosh ; Divakar Hemadri ; Aniket Sanyal ; Tamilselvan Ramasamy Periyasamy ; Thaha Jamal Rasool
• 关键词：FMD virus ; Serotype A ; Genotype differentiation ; RT-PCR ; Sandwich ELISA
• 刊名：Journal of Virological Methods
• 出版年：2007
• 出版时间：July 2007
• 年：2007
• 卷：143
• 期：1
• 页码：117-121
• 全文大小：191 K

文摘

Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.