High efficient chromogenic catalysis of tetramethylbenzidine with horseradish peroxidase immobilized magnetic nanoparticles

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High efficient chromogenic catalysis of tetramethylbenzidine with horseradish peroxidase immobilized magnetic nanoparticles

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作者：S. Kim¹ ; J. Lee¹ ; S. Jang ; H. Lee ; D. Sung ; J.H. Chang ; ^{jhchang@kicet.re.kr" class="auth_mail" title="E-mail the corresponding author}
关键词：Bioimmobilization ; Biocatalysis ; Enzyme activity ; Absorption ; Horseradish peroxidase ; Magnetic nanoparticles
刊名：Biochemical Engineering Journal
出版年：2016
出版时间：15 January 2016
年：2016
卷：105
期：part_PB
页码：406-411
全文大小：1250 K

文摘

•: The binding capacity of HRP onto Si-MNPs is 5 ± 1.5 μg per 1 mg of Si-MNPs.
•: Chromogenic catalysis of TMB was evaluated by HRP@Si-MNPs and free HRP.
•: Apparent kinetic constant (V_max) of immobilized HRP was 260 μmol/(min mg protein).
•: The obtained V_max was 88-folds higher than the free HRP.

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