Optical fluctuation microscopy based on calculating local entropy values

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• 作者：Idir Yahiat猫ne ; Simon Hennig ; Thomas Huser
• 关键词：SNR ; signal-to-noise ratio ; ECI ; entropy-based contrast-enhanced imaging ; QD ; quantum dot ; PSF ; pointspread function
• 刊名：Chemical Physics Letters
• 出版年：5 November, 2013
• 年：2013
• 卷：587
• 期：Complete
• 页码：1-6
• 全文大小：1510 K

文摘

We demonstrate a novel and easy-to-use method to dramatically reduce noise and background contributions in advanced fluorescence microscopy experiments. The underlying idea is that the entropy value increases for systems with a large number of accessible energy states. Intensity fluctuations originating from photophysical or photochemical effects lead to an increased information content. Calculating the pixel-wise entropy value results in an enhancement of the signal-to-noise ratio by a factor of 90-100. Comparing ECI (entropy-based contrast-enhanced imaging) to superresolution methods such as STORM and SOFI, we find that this technique also bears substantial potential for enhancing fluctuation-based superresolution microscopies.