A Single-Cell Roadmap of Lineage Bifurcation in Human ESC Models of Embryonic Brain Development

详细信息    查看全文

• 作者：Zizhen Yao¹ ; ⁵ ; John K. Mich¹ ; ⁵ ; Sherman Ku¹ ; ⁵ ; Vilas Menon¹ ; ⁵ ; Anne-Rachel Krostag¹ ; Refugio A. Martinez¹ ; Leon Furchtgott² ; Heather Mulholland¹ ; Susan Bort¹ ; Margaret A. Fuqua¹ ; Ben W. Gregor¹ ; Rebecca D. Hodge¹ ; Anu Jayabalu¹ ; Ryan C. May¹ ; Samuel Melton³ ; Angelique M. Nelson¹ ; N. Kiet Ngo¹ ; Nadiya V. Shapovalova¹ ; Soraya I. Shehata¹ ; Michael W. Smith¹ ; Leah J. Tait¹ ; Carol L. Thompson¹ ; Elliot R. Thomsen¹ ; Chaoyang Ye¹ ; Ian A. Glass⁴ ; Ajamete Kaykas¹ ; Shuyuan Yao¹ ; John W. Phillips¹ ; Joshua S. Grimley¹ ; ^{grimley@gmail.com} ; Boaz P. Levi¹ ; ⁶ ; ^{boazl@alleninstitute.org} ; Yanling Wang¹ ; ^{ywseattle@gmail.com} ; Sharad Ramanathan¹ ; ² ; ³ ; ^{sharad@post.harvard.edu}
• 关键词：human embryonic stem cells ; single cell RNA-seq ; lineage ; neurogenesis
• 刊名：Cell Stem Cell
• 出版年：2017
• 出版时间：5 January 2017
• 年：2017
• 卷：20
• 期：1
• 页码：120-134
• 全文大小：15869 K
• 卷排序：20

文摘

Single-cell RNA-seq of differentiating hESCs reveals a multitude of neural cell types hESC-derived cells exhibit a range of early brain regional identities Bayesian analyses reconstruct, and clonal analyses confirm, lineage relationships Wnt/β-catenin signaling controls bifurcations between forebrain and mid/hindbrain lineages