Evaluation of the PowerPlex Fusion System in a sample from East Timor

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• 作者：L. Souto ; F. Tavares ; H. Moreira ; F. Corte-Real
• 关键词：PowerPlex® ; Fusion ; East Timor population ; Autosomal STR ; Allele frequency
• 刊名：Forensic Science International: Genetics Supplement Series
• 出版年：2013
• 年：2013
• 卷：4
• 期：1
• 页码：e105-e106
• 全文大小：200 K

文摘

East Timor is a country located in Southeast Asia. In this study, we determined allele frequencies and forensic parameters for 24 STR autosomal loci included in the PowerPlex^庐 Fusion System. Autosomal STR data was collected from saliva samples of 100 individuals from East Timor. The amplification of the 24 autosomal STRs was performed using PowerPlex^庐 Fusion System (Promega Corporation) and the amplified products were analysed on 3500 Genetic Analyser using GeneMapper^庐 ID-X 1.2 Software (Applied Biosystems). All the analysed loci meet Hardy-Weinberg equilibrium after Bonferroni correction. In our samples, we found 鈥渙ff-ladder鈥?alleles (D2S441, Penta E and FGA locus) confirmed by reamplification, amplification with others kits and sequenced when justified.