High performance liquid chromatographic methods for the determination of aripiprazole with ultraviolet detection in rat plasma and brain: Application to the pharmacokinetic study

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• 作者：Shimokawa ; Yoshihiko ; Akiyama ; Hitoshi ; Kashiyama ; Eiji ; Koga ; Toshihisa ; Miyamoto ; Gohachiro
• 关键词：Aripiprazole ; OPC-14597 ; Abilify™
• 刊名：Jnl. of Chromatography B, Anl.Tech. in the Biomed.& Life Sci
• 出版年：2005
• 出版时间：July 5, 2005
• 年：2005
• 卷：821
• 期：1
• 页码：8-14
• 全文大小：128 K

文摘

High performance liquid chromatographic (HPLC) methods were validated for the determination of aripiprazole (OPC-14597, Abilify™) in rat plasma and brain. Separation was by Nova-pak phenyl column; flow rate, 1.0 ml/min; mobile phase, acetonitrile–methanol–20 mM sodium sulfate–acetic acid (27:25:48:1, v/v/v/v); UV detection at 254 nm. Reproducibility in plasma and brain showed excellent precision (within 7.8 and 10.6 % ) and accuracy (96.0–102.4 % and 99.0–108.7 % ) with calibration curve ranges 10.0–2000 ng/ml and 30.0–6000 ng/g, respectively. Validated HPLC methods were successfully applied to pharmacokinetic study of aripiprazole in rats, demonstrating brain concentrations after oral administration five times higher than plasma concentrations.