Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering

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• 作者：Feng Ge ; Xu Chen ; Xin Wang ; Xuewei Liao ; Yiying Jiao ; Qing Hong ; Longjiang Zhang ; Jun Wu
• 关键词：¦Ã-Hexachlorocyclohexane ; 2 ; 4 ; 5-Trichlorophenoxyacetic acid ; 2 ; 5-Dichlorohydroquinone ; Homologous recombination ; Mineralization
• 刊名：Journal of Biotechnology
• 出版年：2013
• 出版时间：20 July, 2013
• 年：2013
• 卷：166
• 期：4
• 页码：187-191
• 全文大小：1256 K

文摘

The gene cassette encoding for TftAB and TftCD proteins was integrated into the 16srDNA gene of the ¦Ã-hexachlorocyclohexane (¦Ã-HCH) mineralizing strain Sphingobium sp. BHC-A by homologous recombination. The recombinant ¦Ã-HCH mineralizing strain may degrade 2,4,5-trichlorophenoxyacetic acid at a rate of 250 nmol mg [protein]^?1 h^?1, and the generated intermediate 2,5-dichlorohydroquinone may be further mineralized through ¦Ã-HCH downstream degradation pathway.