Chronopotentiometric sensing of specific interactions between lysozyme and the DNA aptamer

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• 作者：Veronika Ostatn&aacute ; ^a ; ^{ostatna@ibp.cz} ; Veronika Kasalov&aacute ; -Vargov&aacute ; ^a ; L&aacute ; szló ; Ké ; kedy-Nagy^b ; Hana Černock&aacute ; ^a ; Elena E. Ferapontova^b
• 关键词：DNA-protein interactions ; Lysozyme ; Aptamer ; Constant current chronopotentiometric stripping ; Thiol-modified mercury electrodes ; Catalytic hydrogen evolution
• 刊名：Bioelectrochemistry
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：114
• 期：Complete
• 页码：42-47
• 全文大小：525 K
• 卷排序：114

文摘

Lysozyme-DNA-aptamer complexes were electrochemically detected at Hg electrodes. Specific and non-specific lysozyme-aptamer complexes were discriminated. Modification with thioglycolic acid promoted adsorption of complexes on the Hg surface. Lysozyme-DNA complexes adsorbed on Hg disintegrated at negative potentials. Constant current chronopotentiometry allows analysis of protein-DNA interactions.