Multiple-locus variable number of tandem repeat analysis as a tool for molecular epidemiology of botulism: The Italian experience

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• 作者：Fabrizio Anniballi^a ; ¹ ; ^{fabrizio.anniballi@iss.it} ; Silvia Fillo^b ; ¹ ; Francesco Giordani^b ; ¹ ; Bruna Auricchio^a ; Domenico Azarnia Tehran^b ; Enrica di Stefano^b ; Giuseppina Mandarino^c ; Dario De Medici^a ; Florigio Lista^b
• 关键词：AFLP ; Amplified Fragment Length Polymorphism ; BoNT ; Botulinum neurotoxin ; MLST ; Multi-Locus Sequence Typing ; MLVA ; Multiple Locus Variable-number tandem repeat Analysis ; NRCB ; National Reference Centre for Botulism ; PFGE ; Pulsed Field Gel Electrophoresis ; UPGMA ; Unweight Pair Group Method with Arithmetic mean
• 刊名：Infection, Genetics and Evolution
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：46
• 期：Complete
• 页码：28-32
• 全文大小：258 K
• 卷排序：46

文摘

MLVA is a suitable tool for molecular epidemiology and supporting the surveillance system. MLVA could be performed from enrichment broths and directly from foods and clinical specimens. Sub-typing should be carried out in real-time when the sample is still available in laboratory.