Differences among Passiflora species for pollen amount and viability were detected.
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The pollen/ovule ratio and in vivo pollination were useful for reproductive analysis.
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Cross-pollination presented higher fertilization percentage than self-pollination.
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Depending on the species, self-incompatibility occurred in different floral structures.
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Regions of self-incompatibility in the pistil were detected by fluorescence analysis.
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