cAMP-dependent protein kinase type RI is found in clusters in the rat detergent-insoluble neuronal fraction

详细信息    查看全文

• 作者：Mucignat-Caretta ; Carla ; Conti ; Virna ; Caretta ; Antonio
• 关键词：Protein kinase A ; Brain ; Fluorescent cyclic nucleotides ; Immunohistochemistry ; Rat ; Cytoskeleton
• 刊名：Neuroscience Letters
• 出版年：1999
• 出版时间：September 24, 1999
• 年：1999
• 卷：273
• 期：1
• 页码：1-4
• 全文大小：90.1 K

文摘

Different types of cAMP dependent regulatory subunits have been characterized in the mammalian brain: RI α and β, RII α and β. The subcellular distribution of RI subunits has been examined in the rat brain. Partial amino acid sequencing of tryptic fragments from the Triton insoluble pellet of the rat brain shows that cAMP dependent regulatory subunits type RI α are found in this fraction. Immunohistochemistry shows that Triton-insoluble RI subunits are concentrated to form clusters and this distribution is distinct from RII subunits. Immunohistochemistry and fluorescent cAMP labeling show that the clusters bind fluorescent cAMP analogues. These results suggest that the high local concentration of RI subunits can modulate cAMP distribution among different cellular compartments.