Psidium guajava L. leaves as source of proanthocyanidins: Optimization of the extraction method by RSM and study of the degree of polymerization by NP-HPLC-FLD-ESI-MS

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• 作者：Elixabet Dí ; az-de-Cerio^a ; ^b ; Federica Pasini^c ; Vito Verardo^d ; ^{vito.verardo@unibo.it} ; Alberto Ferná ; ndez-Gutié ; rrez^a ; ^b ; Antonio Segura-Carretero^a ; ^b ; Maria Fiorenza Caboni^c ; ^e
• 关键词：Guava (Psidium guajava L.) leaves ; Proanthocyanidins oligomers ; Ultrasound assisted extraction ; Response surface methodology ; NP-HPLC-FLD-ESI-MS
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：133
• 期：Complete
• 页码：1-7
• 全文大小：1409 K
• 卷排序：133

文摘

Ultrasound extraction of proanthocyanidins from Psidium guajava was established. Best conditions: 50% acetone/water (v/v), 48 °C, 30 min, and 0% acetic acid (v/v). Low oxidized guava leaves reported the greatest procyanidin content by NP-HPLC-FLD. High degree of polymerization (DP1-DP13) has been found in guava leaves.