GABAergic neurons in the ventrolateral subnucleus of the nucleus tractus solitarius are in contact with Kölliker-Fuse nucleus neurons projecting to the rostral ventral respiratory group and phren

详细信息    查看全文

• 作者：Shigefumi Yokota ; Toshiko Tsumori ; Tatsuro Oka ; Sawako Nakamura ; Yukihiko Yasui
• 关键词：Kö ; lliker-Fuse nucleus ; Nucleus tractus solitarius ; Ventral respiratory group ; GABAergic ; Glutamic acid decarboxylase 67 ; Rat
• 刊名：Brain Research
• 出版年：2008
• 出版时间：4 September 2008
• 年：2008
• 卷：1228
• 期：Complete
• 页码：113-126
• 全文大小：2509 K

文摘

After ipsilateral injections of biotinylated dextran amine (BDA) into the ventrolateral subnucleus of the nucleus tractus solitarius (vlNTS) and Fluoro-gold (FG) into the rostral ventral respiratory group (rVRG) region or into the phrenic nucleus (PhN) region in the rat, an overlapping distribution of BDA-labeled axon terminals and FG-labeled neurons was found in the Kölliker-Fuse (KF) nucleus ipsilateral to the injection sites. Using retrograde tracing combined with immunohistochemistry for glutamic acid decarboxylase isoform 67 (GAD67), we indicated that as many as 40 % of the vlNTS neurons projecting to the KF were immunoreactive for GAD67. Using a combination of anterograde and retrograde tracing techniques, and immunohistochemistry for GAD67, we further demonstrated that the vlNTS axon terminals with GAD67 immunoreactivity established close contact to the rVRG- or PhN-projecting KF neurons. The present results suggest that GABAergic vlNTS fibers may exert inhibitory influences on the rVRG- as well as PhN-projecting KF neurons and these circuits may be involved in the respiratory reflexes such as the Hering–Breuer reflex.