Assessment of SOb>3b> micro-thermal explosion pretreatment on wheat straw was conducted.
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77.5% of lignin in wheat straw was removed after pretreatment.
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Cellulose and hemicellulose were almost not to be degraded to form inhibitor.
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The highest saccharification rate of the pretreated wheat straw was 89.3%.
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Hydration of SOb>3b> and esterification of lignin were the key factors of the process.
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