Rapid separation and identification of multiple constituents in Danhong Injection by ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry

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• 作者：Qun-Qun ZHANG ; Xin DONG ; Xin-Guang LIU ; Wen GAO ; Ping LI ; ^{liping2004@126.com" class="auth_mail" title="E-mail the corresponding author} ; Hua YANG ; ^{yanghuacpu@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：UHPLC-ESI-QTOF/MS ; Danhong Injection ; Radix Salviae Miltiorrhizae ; Flos Carthami tinctorii ; TCM ; Mass spectrometry
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：14
• 期：2
• 页码：147-160
• 全文大小：1981 K

文摘

To characterize and identify multiple constituents in Danhong injection (DHI), a fast ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QTOF/MS) method was established and validated in the present study. A total of 63 compounds, including 33 phenolic acids, 2 C-glycosyl quinochalcones, 6 flavonoid O-glycosides, 4 iridoid glycosides, 6 organic acids, 5 amino acids, and 3 nucleosides, were identified or tentatively characterized. In conclusion, the UHPLC-ESI-QTOF/MS method is useful and efficient for in-depth structural elucidation of chemical compounds in complex matrices of herbal medicines such as DHI.