A biosensing application based on quenching the enhanced electrochemiluminescence of poly[tris(N-bipyridylethyl)pyrrole] ruthenium(II) film by Au nanoparticles

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• 作者：Jiangyan Xia^a ; ^b ; Shou-Nian Ding^b ; ^{snding@seu.edu.cn} ; Bu-Hong Gao^b ; Yue-Ming Sun^b ; Yi-Hong Wang^b ; Serge Cosnier^c ; Xinli Guo^d
• 关键词：Electrochemiluminescence ; Tris(2 ; 2&prime ; -bipyridyl)ruthenium(II) derivative ; Polypyrrole ; Gold nanoparticles ; Biosensor
• 刊名：Journal of Electroanalytical Chemistry
• 出版年：2013
• 出版时间：1 March, 2013
• 年：2013
• 卷：692
• 期：Complete
• 页码：60-65
• 全文大小：827 K

文摘

A new stable solid-state electrochemiluminescence (ECL) sensor was fabricated by electropolymerizing tris ((4-(2-pyrrol-1-ylethyl)-4¡ä-methyl-2,2¡ä-bipyridine) ruthenium(II) complex (denoted as Ru(L)₃) upon glassy carbon electrode (GCE). Meanwhile, 3.5-fold enhanced ECL intensity of polyRu(L)₃/GCE was obtained in H₂O/CH₃CN solvent instead of H₂O. Interestingly, an obvious ECL quenching was observed by introducing gold nanoparticles onto the polymer film. Atomic force microscope (AFM) was used to characterize the obtained polyRu(L)₃ film and nanogold seeds/polyRu(L)₃ film morphologies. The electrochemical and ECL behaviors were investigated by cyclic voltammetry. Based on the efficient quenching, a new glucose ECL biosensor as a model was accomplished by in situ growing-up gold seeds in solution induced by biologically generated H₂O₂. The linear range to detect glucose is from 5.0 ¡Á 10^?5 M to 2.0 ¡Á 10^?3 M with the limit of detection of 2.5 ¡Á 10^?5 M.