Early detection of Trichinella spiralis DNA in the feces of experimentally infected mice by using PCR

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• 作者：Xiao Lin Liu ; Hua Nan Ren ; Ya Li Shi ; Chen Xi Hu ; Yan Yan Song ; Jiang Yang Duan ; Hui Ping Zhang ; Xin Rui Du ; Ruo Dan Liu ; Peng Jiang ; Zhong Quan Wang ; ^{wangzq@zzu.edu.cn} ; Jing Cui ; ^{cuij@zzu.edu.cn}
• 关键词：Trichinella spiralis ; DNA ; Feces ; PCR ; Early diagnosis
• 刊名：Acta Tropica
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：166
• 期：Complete
• 页码：351-355
• 全文大小：1164 K
• 卷排序：166

文摘

T. spiralis DNA in feces of mice infected with 100 or 300 larvae was assayed by PCR. The sensitivity of PCR was 0.016 larvae in feces. No cross-reactivity was observed with the DNA of other intestinal helminths. T. spiralis DNA was detected in 100% (12/12) of the feces of infected mice at 3 hpi. Detection of fecal DNA might be useful for early diagnosis of Trichinella infection.