A rapid and sensitive UHPLC-MS/MS method for quantification of 83b1 in plasma and its application to bioavailability study in rats

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• 作者：Dingsheng Wen^a ; ^{dswen168@163.com} ; Jing Guo^b ; ^{1043397061@qq.com} ; Fulin Jiang^a ; ^{jflzd163@163.com} ; Caishun Huang^a ; ^{549567264@qq.com} ; Zhenzhen Zhao^a ; ^{yqnlcc77@126.com} ; Gui Lu^b ; ^{lugui@mail.sysu.edu.cn} ; Jiangying Chen^a ; ^{chenjiangying222@163.com} ; Liuyun Qin^a ; Zhangwei Li^a ; ^{395024754@qq.com} ; Xueding Wang^a ; ^{wangxd@mail.sysu.edu.cn} ; Zhuoan Deng^c ; Min Huang^a ; ^{huangmin@mail.sysu.edu.cn} ; Chan Albert Sun Chi^b ; ^{chenxz3@mail.sysu.edu.cn} ; Tang Johnny Cheuk On^c ; ^{dr-johnny.tang@polyu.edu.hk} ; Guoping Zhong^a ; ^{zhonggp@mail.sysu.edu.cn}
• 关键词：UHPLC&ndash ; MS/MS ; ultra-high performance liquid chromatography tandem mass spectrometry ; ESCC ; esophageal squamous cell carcinoma ; PPAR ; peroxisome proliferator-activated receptor ; CDDP ; cisplatin ; COX-2 ; cyclooxygenase-2 ; PGH ; prostaglandin H ; MTS ; 3-(4 ; 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfohenyl)-2H-tetrazolium ; MTBE ; methyl tert-butyl ether ; SRM ; selection reaction monitoring ; QC ; quality control ; Tmax ; Time to maximum plasma concentration ; Cmax ; maximum of plasma conc
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：71-76
• 全文大小：797 K
• 卷排序：134

文摘

The compound 83b1 has the potential to develop as a novel anti- esophageal cancer drug for its high anti-tumor activity and low cytotoxicity. [J1] A simple, rapid and sensitive method for determination of 83b1 in rat plasma using UHPLC–MS/MS was developed and validated for the first time. The validated method was employed to study the bioavailability of 83b1 in rat by dosing with intravenous injection (1 mg/kg) and gavage (10 mg/kg).