Suppression of SERPINA1-albumin complex formation by galectin-3 overexpression leads to paracrine growth promotion of chronic myelogenous leukemia cells

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• 作者：Ryuko Nakayama ; Junya Kuroda ; Nobuko Taniyama ; Mio Yamamoto-Sugitani ; Sayori Wada ; Miki Kiyota ; Shinsuke Mizutani ; Yoshiaki Chinen ; Yosuke Matsumoto ; Hisao Nagoshi ; Yuji Shimura ; Tsutomu Kobayashi ; Shigeo Horiike ; Kenji Sato ; Masafumi Taniwaki
• 关键词：Galectin-3 ; Chronic myelogenous leukemia ; SERPINA1 ; Bone marrow stromal cell
• 刊名：Leukemia Research
• 出版年：January, 2014
• 年：2014
• 卷：38
• 期：1
• 页码：103-108
• 全文大小：1507 K

文摘

Galectin-3 is induced in chronic myelogenous leukemia (CML) cells by co-culture with bone marrow stromal cells, making paracrine growth promotion of CML cells in conditioned medium (CM) from galectin-3 overexpressing CML cells more potent. We used gel filtration chromatography to demonstrate that the bovine SERPINA1-fetal bovine serum albumin (BSA) complex was specifically suppressed in CM from galectin-3 overexpressing cells. The SERPINA1-BSA complex as well as human plasma SERPINA1 inhibited the growth of CML cells, while exogenous galectin-3 partly offset this effect. These findings suggest that galectin-3 overexpression promotes paracrine growth of CML cells by interfering with the action of the growth inhibitory SERPINA1-albumin complex.