Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF<sub>6</sub>-hydrolyzate media

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Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF₆-hydrolyzate media

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作者：Yu-Cai He^a ; ^b ; ^c ; ^{heyucai2001@163.com" class="auth_mail" title="E-mail the corresponding author} ; ^{yucaihe@cczu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Zhi-Cheng Tao^a ; Jun-Hua Di^a ; Liang Chen^a ; Lin-Bing Zhang^a ; Dan-Ping Zhang^a ; Gang-Gang Chong^a ; Feng Liu^a ; Yun Ding^a ; Chun-Xia Jiang^a ; Cui-Luan Ma^a ; ^c
关键词：Rice straw ; Dilute NaOH pretreatment ; Ethyl 4-chloro-3-oxobutanoate ; Ethyl (R)-4-chloro-3-hydroxybutanoate ; E. coli CCZU-A13
刊名：Bioresource Technology
出版年：2016
出版时间：August 2016
年：2016
卷：214
期：Complete
页码：411-418
全文大小：1638 K

文摘

•: Hydrolyzates promoted initial reaction rate and intracellular NADH content.
•: Emulsifier OP-10 (20 mM) increased the initial reaction rate of whole cells.
•: Hp-β-cyclodextrin (0.010 mol Hp-β-CD/mol COBE) increased the initial reaction rate.
•: Effective biosynthesis of (R)-CHBE (800 mM) was in [Bmim]PF₆-hydrolyzate media.