Application of retrovirus-mediated expression cloning for receptor screening of a parasite

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• 作者：Kyousuke Kobayashi ; Kentaro Kato ; Tatsuki Sugi ; Daisuke Yamane ; Masayuki Shimojima ; Yukinobu Tohya ; Hiroomi Akashi
• 刊名：Analytical Biochemistry
• 出版年：2009
• 出版时间：1 June 2009
• 年：2009
• 卷：389
• 期：1
• 页码：80-82
• 全文大小：263 K

文摘

Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.