Overexpression of Midkine promotes the viability of BA/F3 cells

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• 作者：Yang Wang ; Haiyan Xing ; Zheng Tian ; Kejing Tang ; Jiying Wang ; Zhifang Xu ; Qing Rao ; Min Wang ; Jianxiang Wang
• 关键词：Midkine ; Acute leukemia ; BA/F3 ; Transformation ; Proliferation ; Apoptosis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：3 July 2009
• 年：2009
• 卷：384
• 期：3
• 页码：341-346
• 全文大小：418 K

文摘

Midkine (MK), a heparin-binding growth factor, has been reported to be overexpressed in a variety of human solid tumors. In the previous study, we found that MK was overexpressed in bone marrow samples derived from acute leukemia (AL) patients. To elucidate the role of MK, we stably transfected MK in IL-3-dependent BA/F3 cells. The results indicated that the capacity of proliferation and colony formation was significantly increased in the MK-transfected subclones than in the empty vector-transfected subclones. MK potentiated proliferation of BA/F3 cells by promoting cell cycle progression. Apoptosis assays showed a remarkable reduction of apoptosis in MK expressing subclones. Exogenous MK could induce the phosphorylation of Raf-1, and inhibit the expression of Bax in BA/F3 cells. These results indicate that MK might be involved in the pathogenesis of leukemia and could be taken as an ideal diagnostic marker and molecular target for the treatment of acute leukemia.