Selective enrichment of phosphopeptides by titania nanoparticles coated magnetic carbon nanotubes

详细信息    查看全文

• 作者：Yinghua Yan ; Zhifang Zheng ; Chunhui Deng ; Xiangmin Zhang ; Pengyuan Yang
• 关键词：MagCNTs@TiO2 composites ; Selective enrichment ; Phosphopeptides ; Mass spectrometry analysis
• 刊名：Talanta
• 出版年：15 January, 2014
• 年：2014
• 卷：118
• 期：Complete
• 页码：14-20
• 全文大小：3305 K

文摘

Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for mass spectrometry (MS)-based phosphoproteomics. In this work, for the first time, titania nanoparticles coated magnetic carbon nanotubes (denoted as MagCNTs@TiO₂ composites) were synthesized through a facile but effective solvothermal reaction for selective enrichment of phosphopeptides. The MagCNTs@TiO₂ material demonstrated low limit of detection (20 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein 尾-casein with molar ratios of BSA/尾-casein up to 200:1. In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. Experimental results demonstrated that the MagCNTs@TiO₂ composites showed excellent potential for the selective enrichment of phosphopeptides for MS analysis.