Study of the Interaction between Actin and Antitumor Substance Aplyronine A with a Novel Fluorescent Photoaffinity Probe

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• 作者：Takeshi Kuroda ; Kiyotake Suenaga ; Akira Sakakura ; Tomohisa Handa ; Kazuhito Okamoto ; and Hideo Kigoshi
• 刊名：Bioconjugate Chemistry
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：17
• 期：2
• 页码：524 - 529
• 全文大小：176K
• 年卷期：v.17,no.2(March 2006)
• ISSN：1520-4812

文摘

The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marineorigin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chainportion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detectinggroup were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin wassuccessfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the firstchemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.