Advanced Substrate Fabrication for Cell Microarrays

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• 作者：Andrew L. Hook*^† ; ^‡ ; ; Helmut Thissen^‡ ; ; Nicolas H. Voelcker^† ;
• 刊名：Biomacromolecules
• 出版年：2009
• 出版时间：March 9, 2009
• 年：2009
• 卷：10
• 期：3
• 页码：573-579
• 全文大小：282K
• 年卷期：v.10,no.3(March 9, 2009)
• ISSN：1526-4602

文摘

The fabrication and characterization of chemical patterns using a technique that can be readily integrated with methods currently used for the formation of microarrays is presented. A high density poly(ethylene glycol) coating was deposited on glass slides as a background exhibiting low cell attachment properties. Phenylazide modified polymers were then printed on this background. UV irradiation of these polymer arrays resulted in the cross-linking of the polymer spots and their covalent attachment to the surface. Cell attachment was shown to follow the resultant surface chemistry pattern. Furthermore, the use of a robotic contact printer enabled the facile deposition of DNA microarrays on top of and aligned with the polymer microarrays. A transfected cell microarray was generated in this way, demonstrating not only the ability of this platform to limit cell attachment to specific regions, but the suitability for chip-based functional genomics, in particular, and high density cell assays in general.