Impact of Autosomal Recessive Juvenile Parkinson鈥檚 Disease Mutations on the Structure and Interactions of the Parkin Ubiquitin-like Domain

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• 作者：Susan S. Safadi ; Kathryn R. Barber ; Gary S. Shaw
• 刊名：Biochemistry
• 出版年：2011
• 出版时间：April 5, 2011
• 年：2011
• 卷：50
• 期：13
• 页码：2603-2610
• 全文大小：892K
• 年卷期：v.50,no.13(April 5, 2011)
• ISSN：1520-4995

文摘

Autosomal recessive juvenile parkinsonism (ARJP) is an early onset familial form of Parkinson鈥檚 disease. Approximately 50% of all ARJP cases are attributed to mutations in the gene park2, coding for the protein parkin. Parkin is a multidomain E3 ubiquitin ligase with six distinct domains including an N-terminal ubiquitin-like (Ubl) domain. In this work we examined the structure, stability, and interactions of the parkin Ubl domain containing most ARJP causative mutations. Using NMR spectroscopy we show that the Ubl domain proteins containing the ARJP substitutions G12R, D18N, K32T, R33Q, P37L, and K48A retained a similar three-dimensional fold as the Ubl domain, while at least one other (V15M) had altered packing. Four substitutions (A31D, R42P, A46P, and V56E) result in poor folding of the domain, while one protein (T55I) showed evidence of heterogeneity and aggregation. Further, of the substitutions that maintained their three-dimensional fold, we found that four of these (V15M, K32T, R33Q, and P37L) lead to impaired function due to decreased ability to interact with the 19S regulatory subunit S5a. Three substitutions (G12R, D18N, and Q34R) with an uncertain role in the disease did not alter the three-dimensional fold or S5a interaction. This work provides the first extensive characterization of the structural effects of causative mutations within the ubiquitin-like domain in ARJP.