Protein−Nanoparticle Interaction: Identification of the Ubiquitin−Gold Nanoparticle Interaction Site

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• 作者：Luigi Calzolai ; Fabio Franchini ; Douglas Gilliland ; Franois Rossi
• 刊名：Nano Letters
• 出版年：2010
• 出版时间：August 11, 2010
• 年：2010
• 卷：10
• 期：8
• 页码：3101-3105
• 全文大小：328K
• 年卷期：v.10,no.8(August 11, 2010)
• ISSN：1530-6992

文摘

We demonstrate that it is possible to identify the protein−nanoparticle interaction site at amino acid scale in solution. Using NMR, chemical shift perturbation analysis, and dynamic light scattering we have identified a specific domain of human ubiquitin that interacts with gold nanoparticles. This method allows a detailed structural analysis of proteins absorbed onto surfaces of nanoparticles in physiological conditions and it will provide much needed experimental data for better modeling and prediction of protein−nanoparticle interactions.

Keywords:

Gold nanoparticles; NMR; protein−nanoparticle interactions; chemical shift mapping