Synthesis of a New Conjugated Polymer for DNA Alkylation and Gene Regulation

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• 作者：Chenyao Nie ; Chunlei Zhu ; Liheng Feng ; Fengting Lv ; Libing Liu ; Shu Wang
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：June 12, 2013
• 年：2013
• 卷：5
• 期：11
• 页码：4549-4554
• 全文大小：316K
• 年卷期：v.5,no.11(June 12, 2013)
• ISSN：1944-8252

文摘

A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs).

Keywords:

conjugated polymers; DNA alkylation; plasmid; GFP; gene regulation; protein expression