A Photocleavable Rapamycin Conjugate for Spatiotemporal Control of Small GTPase Activity

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• 作者：Nobuhiro Umeda ; Tasuku Ueno ; Christopher Pohlmeyer ; Tetsuo Nagano ; Takanari Inoue
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：January 12, 2011
• 年：2011
• 卷：133
• 期：1
• 页码：12-14
• 全文大小：182K
• 年卷期：v.133,no.1(January 12, 2011)
• ISSN：1520-5126

文摘

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.