X-ray Structure Analysis of Free-Standing Lipid Membranes Facilitated by Micromachined Apertures

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• 作者：Andr&eacute ; Beerlink ; P.-J. Wilbrandt ; E. Ziegler ; D. Carbone ; T. H. Metzger ; Tim Salditt
• 刊名：Langmuir
• 出版年：2008
• 出版时间：May 6, 2008
• 年：2008
• 卷：24
• 期：9
• 页码：4952 - 4958
• 全文大小：540K
• 年卷期：v.24,no.9(May 6, 2008)
• ISSN：1520-5827

文摘

Silicon and Teflon substrates have been structured by wet etching and a focused ion beam (FIB) to obtain verydefined, clean apertures. Planar, free-standing lipid membranes (black lipid membranes (BLM)) with enhanced long-term stability have been prepared on these apertures by the methods of Montal and Müller^1,2 as well as Müller andRudin.³ The stability and geometric control enables the use of X-ray analysis of free-standing single bilayers. Withthe presented setup, simultaneous structural and electrophysiological measurements will become feasible.