Testing Oligothiophene Fluorophores under Physiological Conditions. Preparation and Optical Characterization of the Conjugates of Bovine Serum Albumin with Oligothiophene N-Hydroxysuccinimidyl

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• 作者：Massimo Zambianchi ; Andrea Barbieri ; Alfredo Ventola ; Laura Favaretto ; Cristian Bettini ; Matteo Galeotti ; Giovanna Barbarella
• 刊名：Bioconjugate Chemistry
• 出版年：2007
• 出版时间：May 2007
• 年：2007
• 卷：18
• 期：3
• 页码：1004 - 1009
• 全文大小：101K
• 年卷期：v.18,no.3(May 2007)
• ISSN：1520-4812

文摘

Bovine serum albumin (BSA) was reacted with linear and newly synthesized branched oligothiopheneN-hydroxysuccinimidyl ester fluorophores (TSEs) in moderately basic carbonate buffer solution. Optically stableBSA-TSE conjugates were obtained with a degree of labeling depending on experimental conditions. Conjugateswith high fluorophore to BSA ratios (F/BSA = 8) displayed fluorescence quantum yields in the range of 10-30% in water at pH = 7.2, comparable to the quantum yield (25%) of the BSA-FITC conjugate prepared underthe same conditions and with the same degree of labeling.