Sensitive Insulin Detection based on Electrogenerated Chemiluminescence Resonance Energy Transfer between Ru(bpy)32+ and Au Nanoparticle-Doped β-Cyclodextrin-Pb (II) Metal–Organic Framework

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• 作者：Hongmin Ma ; Xiaojian Li ; Tao Yan ; Yan Li ; Haiyang Liu ; Yong Zhang ; Dan Wu ; Bin Du ; Qin Wei
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：April 27, 2016
• 年：2016
• 卷：8
• 期：16
• 页码：10121-10127
• 全文大小：427K
• 年卷期：0
• ISSN：1944-8252

文摘

A novel sandwich-type electrochemiluminescent (ECL) biosensor based on ECL resonance energy transfer (RET) was developed for the determination of insulin. Au nanoparticle-doped Pb(II)-β-cyclodextrin (Pb-β-CD) metal–organic framework was exploited as an ECL donor and chitosan/Ru(bpy)₃²⁺/silica nanoparticles (CRuSi NPs) were employed as ECL acceptor. Pb-β-CD shows excellent ECL behavior and unexpected reducing ability toward AuCl₄^–. Au nanoparticles could massively form on the surface of Pb-β-CD (Au@Pb-β-CD) without use of any additional reducing agent. In the presence of co-reactant K₂S₂O₈, the ECL emission of Pb-β-CD was enhanced by the formation of Au nanoparticles, which is desirable for the construction of a RET immunosensor. The absorption spectra of the synthesized CRuSi NPs show great overlaps with the ECL spectra of Pb-β-CD. This enabled the ECL-RET by incorporating Au@Pb-β-CD and CRuSi NPs into electrode surfaces. The immunosensor is fabricated using Au@Pb-β-CD as sensing platform and CRuSi NPs as labels of antibodies. After the optimization of determination conditions, a linear response range for insulin from 0.1 pg mL^–1 to 10.0 ng mL^–1 was obtained, and the limit of detection was calculated to be 0.042 pg mL^–1 (S/N = 3). The prepared insulin biosensor displayed excellent stability and good specificity.