High-Affinity Mu Opioid Receptor Ligands Discovered by the Screening of an Exhaustively Stereodiversified Library of 1,5-Enediols

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• 作者：Bryce A. Harrison ; Tiffany Malinky Gierasch ; Claire Neilan ; Gavril W. Pasternak ; and Gregory L. Verdine
• 刊名：Journal of the American Chemical Society
• 出版年：2002
• 出版时间：November 13, 2002
• 年：2002
• 卷：124
• 期：45
• 页码：13352 - 13353
• 全文大小：106K
• 年卷期：v.124,no.45(November 13, 2002)
• ISSN：1520-5126

文摘

In this communication, we report the synthesis of an exhaustively stereodiversified library of 16 1,5-enediols (2) and the screening of these compounds for mu opioid receptor (MOR) binding. The stereochemical configuration of 2 strongly impacted the binding affinity, and (S,S,S,R)-2 exhibited a K_i of 8.8 nM for MOR, comparable to that of endomorphin-2 (K_i = 1.2 nM). Moreover, compounds 2 exhibited 5-86-fold selectivity for MOR over delta opioid receptor (DOR) and 16-150-fold selectivity for MOR over kappa opioid receptor (KOR). Additionally, analogues of 2 were synthesized which showed the importance of the trans olefin for receptor binding but that modifications of the C-terminal amino acid were well tolerated. Ligand 11 is noteworthy because it retains only one of the amide bonds present in 1, but binds MOR with an affinity of 10 nM and 110- and 600-fold selectivity for MOR over DOR and KOR. These results demonstrate the utility of stereochemical diversity in the discovery of bioactive small molecules.