A Tool To Visualize and Evaluate Data Obtained by Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

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• 作者：Xiao-jun Li ; Patrick G. A. Pedrioli ; Jimmy Eng ; Dan Martin ; Eugene C. Yi ; Hookeun Lee ; and Ruedi Aebersold
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：July 1, 2004
• 年：2004
• 卷：76
• 期：13
• 页码：3856 - 3860
• 全文大小：417K
• 年卷期：v.76,no.13(July 1, 2004)
• ISSN：1520-6882

文摘

We present a software tool for visualizing data obtainedfrom analyzing complex peptide mixtures by liquid chromatography (LC) electrospray ionization (ESI) mass spectrometry (MS). The data are represented as a two-dimensional density plot. For experiments employingcollision-induced dissociation (CID), links are embeddedin the image to the CID spectra and the correspondingpeptide sequences that are represented by the respectivefeature. The image provides an intuitive method to evaluate sample quality and the performance of an LC-ESI-MSsystem and can be used to optimize experimental conditions. Local patterns of the image can also be used toidentify chemical contaminants and specific peptide features. Therefore, this software tool may have broadapplication in MS-based proteomics.