Development of Activity-Based Probes for Imaging Human 伪-l-Fucosidases in Cells

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• 作者：Yu-Ling Hsu ; Manjula Nandakumar ; Hsin-Yi Lai ; Tzyy-Chao Chou ; Chi-Yuan Chu ; Chun-Hung Lin ; Lee-Chiang Lo
• 刊名：Journal of Organic Chemistry
• 出版年：2015
• 出版时间：August 21, 2015
• 年：2015
• 卷：80
• 期：16
• 页码：8458-8463
• 全文大小：383K
• ISSN：1520-6904

文摘

We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for 伪-l-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal 伪-l-fucosidase activity in human cells.