Triphenylmethane Dye Activation of Beta-Arrestin

详细信息    查看全文

• 作者：Larry S. Barak ; Yushi Bai ; Joshua C. Snyder ; Jiangbo Wang ; Wei Chen ; Marc G. Caron
• 刊名：Biochemistry
• 出版年：2013
• 出版时间：August 13, 2013
• 年：2013
• 卷：52
• 期：32
• 页码：5403-5414
• 全文大小：782K
• 年卷期：v.52,no.32(August 13, 2013)
• ISSN：1520-4995

文摘

尾-Arrestins regulate G protein-coupled receptor signaling as competitive inhibitors and protein adaptors. Low molecular weight biased ligands that bind receptors and discriminate between the G protein dependent arm and 尾-arrestin, clathrin-associated arm of receptor signaling are considered therapeutically valuable as a result of this distinctive pharmacological behavior. Other than receptor agonists, compounds that activate 尾-arrestins are not available. We show that within minutes of exposure to the cationic triphenylmethane dyes malachite green and brilliant green, tissue culture cells recruit 尾-arrestins to clathrin scaffolds in a receptor-activation independent manner. In the presence of these compounds, G protein signaling is inhibited, ERK and GSK3尾 signaling are preserved, and the recruitment of the beta2-adaptin, AP2 adaptor complex to clathrin as well as transferrin internalization is reduced. Moreover, malachite green binds 尾-arrestin2-GFP coated immunotrap beads relative to GFP only coated beads. Triphenylmethane dyes are FDA approved for topical use on newborns as components of triple-dye preparations and are not approved but used effectively as aqueous antibiotics in fish husbandry. As possible carcinogens, their chronic ingestion in food preparations, particularly through farmed fish, is discouraged in the U.S. and Europe. Our results indicate triphenylmethane dyes as a result of novel pharmacology may have additional roles as 尾-arrestin/clathrin pathway signaling modulators in both pharmacology research and clinical therapy.