Electrotriggered, Spatioselective, Quantitative Gene Delivery into a Single Cell Nucleus by Au Nanowire Nanoinjector

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• 作者：Seung Min Yoo ; Mijeong Kang ; Taejoon Kang ; Dong Min Kim ; Sang Yup Lee ; Bongsoo Kim
• 刊名：Nano Letters
• 出版年：2013
• 出版时间：June 12, 2013
• 年：2013
• 卷：13
• 期：6
• 页码：2431-2435
• 全文大小：338K
• 年卷期：v.13,no.6(June 12, 2013)
• ISSN：1530-6992

文摘

Delivery of bioactive materials into a cell is highly important in the study of cell biology and medical treatments. Ideal nanoinjectors should be able to deliver biomaterials with high spatial resolution while causing minimum cell damage. We developed a Au nanowire (NW) nanoinjector that has the thinnest diameter (100鈥?50 nm) among the DNA delivering devices as well as optimum mechanical properties, minimizing cell damage. Well-defined (111) single-crystalline Au surface and high electric conductivity of a Au NW nanoinjector allow precisely timed and efficient electrochemical release of DNA molecules attached on a Au NW surface. Both linear DNA and plasmid DNA were delivered separately and showed successful expression. The Au NW nanoinjector would find important biomedical applications in the fields such as gene therapy, DNA vaccination, targeted drug delivery, and probe/control of cell signaling events.

Keywords:

Au nanowire; nanoinjector; gene delivery; electrical trigger; DNA