Peptide Photodissociation with 157 nm Light in a Commercial Tandem Time-of-Flight Mass Spectrometer

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• 作者：Liangyi Zhang ; James P. Reilly
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：September 15, 2009
• 年：2009
• 卷：81
• 期：18
• 页码：7829-7838
• 全文大小：337K
• 年卷期：v.81,no.18(September 15, 2009)
• ISSN：1520-6882

文摘

Photodissociation with 157 nm light was implemented in an ABI model 4700 matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF) mass spectrometer for peptide analysis. With a homemade computer program to control the light timing based on the m/z of each precursor ion, the photodissociation setup was seamlessly automated with the mass spectrometer. Peptide photodissociation in this apparatus yielded fragments similar to those observed in previous experiments with a home-built tandem-TOF mass spectrometer. Peptides having arginine at their C-termini yielded high-energy x-, v-, and w- type fragments, while peptides with N-terminal arginine produced many a- and d- type ions. Abundant immonium ions were also generated. High-quality photodissociation spectra were obtained with as little as 5 fmol of peptides. In the analysis of various tryptic peptides, photodissociation provided much more sequence information than the conventional TOF−TOF collision induced dissociation (CID). Because of the high fragmentation efficiency, sensitivity was not sacrificed to achieve this.