Identification of Elaiophylin Derivatives from the Marine-Derived Actinomycete Streptomyces sp. 7-145 Using PCR-Based Screening

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• 作者：Chunyan Wu ; Yi Tan ; Maoluo Gan ; Yiguang Wang ; Yan Guan ; Xinxin Hu ; Hongxia Zhou ; Xiaoya Shang ; Xuefu You ; Zhaoyong Yang ; Chunling Xiao
• 刊名：Journal of Natural Products
• 出版年：2013
• 出版时间：November 22, 2013
• 年：2013
• 卷：76
• 期：11
• 页码：2153-2157
• 全文大小：227K
• 年卷期：v.76,no.11(November 22, 2013)
• ISSN：1520-6025

文摘

A PCR-based genetic screening experiment targeting the dTDP-glucose-4,6-dehydratase gene revealed that a marine sediment-derived strain, Streptomyces sp. 7-145, had the potential to produce glycosidic antibiotics. Chemical investigation of culture extracts of this strain yielded two new 6-deoxyhexose-containing antibiotics, 11鈥?12鈥?dehydroelaiophylin (1) and 11,11鈥?O-dimethyl-14鈥?deethyl-14鈥?methylelaiophylin (2), together with four known elaiophylin analogues (3鈥?b>6). Their structures were determined by extensive NMR, MS, and CD analyses. Compounds 1, 3, 4, and 6 showed good antibacterial activity against methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci pathogens.