Metabolic Engineering of Wheat Provitamin A by Simultaneously Overexpressing CrtB and Silencing Carotenoid Hydroxylase (TaHYD)

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• 作者：Jian Zeng ; Xiatian Wang ; Yingjie Miao ; Cheng Wang ; Mingli Zang ; Xi Chen ; Miao Li ; Xiaoyan Li ; Qiong Wang ; Kexiu Li ; Junli Chang ; Yuesheng Wang ; Guangxiao Yang ; Guangyuan He
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：October 21, 2015
• 年：2015
• 卷：63
• 期：41
• 页码：9083-9092
• 全文大小：450K
• ISSN：1520-5118

文摘

Increasing the provitamin A content in staple crops via carotenoid metabolic engineering is one way to address vitamin A deficiency. In this work a combination of methods was applied to specifically increase 尾-carotene content in wheat by metabolic engineering. Endosperm-specific silencing of the carotenoid hydroxylase gene (TaHYD) increased 尾-carotene content 10.5-fold to 1.76 渭g g^鈥? in wheat endosperm. Overexpression of CrtB introduced an additional flux into wheat, accompanied by a 尾-carotene increase of 14.6-fold to 2.45 渭g g^鈥?. When the 鈥減ush strategy鈥?(overexpressing CrtB) and 鈥渂lock strategy鈥?(silencing TaHYD) were combined in wheat metabolic engineering, significant levels of 尾-carotene accumulation were obtained, corresponding to an increase of up to 31-fold to 5.06 渭g g^鈥?. This is the first example of successful metabolic engineering to specifically improve 尾-carotene content in wheat endosperm through a combination of methods and demonstrates the potential of genetic engineering for specific nutritional enhancement of wheat.