Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography鈥揗ass Spectrometry/Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternativ

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• 作者：Geert Janssens ; Sven Mangelinckx ; Dirk Courtheyn ; Norbert De Kimpe ; Bert Matthijs ; Bruno Le Bizec
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：September 2, 2015
• 年：2015
• 卷：63
• 期：34
• 页码：7574-7581
• 全文大小：341K
• ISSN：1520-5118

文摘

The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring ¹³C/¹²C isotopic ratios of their urinary metabolites. Gas chromatography鈥搈ass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) was used in this study to appraise in a global approach isotopic deviations of two 17尾-testosterone metabolites (17伪-testosterone and etiocholanolone) and one 17尾-estradiol metabolite (17伪-estradiol) together with those of 5-androstene-3尾,17伪-diol as endogenous reference compound (ERC). Intermediate precisions of 0.35鈥? 1.05鈥? 0.35鈥? and 0.21鈥? respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17尾-testosterone propionate and 17尾-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17尾-estradiol treatment up to 24 days. For 17尾-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry (LC-MS/MS) when measuring intact steroid esters in blood and hair.