Double-Nucleus Enhanced Recoupling for Efficient 13C MAS NMR Correlation Spectroscopy of Perdeuterated Proteins

详细信息    查看全文

• 作者：mit Akbey ; Hartmut Oschkinat ; Barth-Jan van Rossum
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：December 2, 2009
• 年：2009
• 卷：131
• 期：47
• 页码：17054-17055
• 全文大小：161K
• 年卷期：v.131,no.47(December 2, 2009)
• ISSN：1520-5126

文摘

The use of both proton and deuterium dipolar coupling networks to obtain efficient ¹³C magic-angle-spinning NMR correlation spectroscopy is introduced. This new strategy aims to improve the efficiency of ¹³C spin diffusion in perdeuterated proteins. The method is called double-nucleus enhanced recoupling (DONER), and it provides significantly improved transfer efficiency for carbon spin diffusion at low proton density. The C_α cross-peak intensity in the DONER experiment is 3 and 5 times stronger than those in conventional radio-frequency-assisted diffusion (RAD) and proton-driven spin diffusion (PDSD) experiments, respectively. Remarkably, the full cross-peak pattern for the aliphatic region of an extensively perdeuterated SH3 protein sample can be obtained using the DONER approach with direct ¹³C excitation.