A New Electrolytic Synthesis Method for Few-Layered MoS2 Nanosheets and Their Robust Biointerfacing with Reduced Antibodies

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• 作者：Manil Kukkar ; Satish K. Tuteja ; Amit L. Sharma ; Vinod Kumar ; Ashok K. Paul ; Ki-Hyun Kim ; Priyanka Sabherwal ; Akash Deep
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：July 6, 2016
• 年：2016
• 卷：8
• 期：26
• 页码：16555-16563
• 全文大小：517K
• 年卷期：0
• ISSN：1944-8252

文摘

We report an efficient method for the synthesis of few-layered MoS₂ nanosheets and demonstrate their application in the label-free detection of the prostate-specific antigen (PSA) cancer marker. As a novel strategy, the electro-dissolution of molybdenum metal sheets in the presence of Na⁺ and S^2– ions led to the formation of Na⁺ intercalated MoS₂. Further exfoliation by ultrasonication yielded the desired formation of few-layered MoS₂ nanosheets. After comprehensive characterization, the synthesized MoS₂ nanosheets were channeled in a field-effect transistor (FET) microdevice. Chemically reduced anti-PSA antibodies were immobilized on the MoS₂ channel above the FET microdevice to construct a specific PSA immunosensor. The antibodies were deliberately reduced to expose the hinge-region disulfide bonds. This approach offered a robust and site-directed immunosensing device through biointerfacing of the sulfhydryl groups (−SH) in the reduced antibody with the surface S atoms of MoS₂. This device was validated as an effective immunosensor with a low detection limit (10^–5 ng/mL) over a wide linear detection range (10^–5 to 75 ng/mL).