TrpB2 Enzymes are O-Phospho-l-serine Dependent Tryptophan Synthases

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• 作者：Florian Busch ; Chitra Rajendran ; Olga Mayans ; Patrick L枚ffler ; Rainer Merkl ; Reinhard Sterner
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：September 30, 2014
• 年：2014
• 卷：53
• 期：38
• 页码：6078-6083
• 全文大小：261K
• ISSN：1520-4995

文摘

The rapid increase of the number of sequenced genomes asks for the functional annotation of the encoded enzymes. We used a combined computational鈥搒tructural approach to determine the function of the TrpB2 subgroup of the tryptophan synthase 尾 chain/尾 chain-like TrpB1鈥揟rpB2 family (IPR023026). The results showed that TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the l-serine dependent synthesis of tryptophan. We found a single residue being responsible for the different substrate specificities of TrpB1 and TrpB2 and confirmed this finding by mutagenesis studies and crystallographic analysis of a TrpB2 enzyme with bound O-phospho-l-serine.