Platelet Proteome Changes Associated with Diabetes and during Platelet Storage for Transfusion

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• 作者：David L. Springer ; John H. Miller ; Sherry L. Spinelli ; Ljiljana Pasa-Tolic ; Samuel O. Purvine ; Donald S. Daly ; Richard C. Zangar ; Shuangshuang Jin ; Neil Blumberg ; Charles W. Francis ; Mark B. Taubman
• 刊名：Journal of Proteome Research
• 出版年：2009
• 出版时间：May 1, 2009
• 年：2009
• 卷：8
• 期：5
• 页码：2261-2272
• 全文大小：228K
• 年卷期：v.8,no.5(May 1, 2009)
• ISSN：1535-3907

文摘

Human platelets play a key role in hemostasis and thrombosis and have recently emerged as key regulators of inflammation. Platelets stored for transfusion produce pro-thrombotic and pro-inflammatory mediators implicated in adverse transfusion reactions. Correspondingly, these mediators are central players in pathological conditions including cardiovascular disease, the major cause of death in diabetics. In view of this, a mass spectrometry based proteomics study was performed on platelets collected from healthy and type-2 diabetics stored for transfusion. Strikingly, our innovative and sensitive proteomic approach identified 122 proteins that were either up- or down-regulated in type-2 diabetics relative to nondiabetic controls and 117 proteins whose abundances changed during a 5-day storage period. Notably, our studies are the first to characterize the proteome of platelets from diabetics before and after storage for transfusion. These identified differences allow us to formulate new hypotheses and experimentation to improve clinical outcomes by targeting “high risk platelets” that render platelet transfusion less effective or even unsafe.