Rational Design of an Optical Sensing System for Multivalent Proteins

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• 作者：Xuedong Song and Basil I. Swanson
• 刊名：Langmuir
• 出版年：1999
• 出版时间：July 6, 1999
• 年：1999
• 卷：15
• 期：14
• 页码：4710 - 4712
• 全文大小：55K
• 年卷期：v.15,no.14(July 6, 1999)
• ISSN：1520-5827

文摘

A generic design principle for detection of multivalent interactions is described. A phospholipid bilayerconsisting of natural and pyrene-derivatized phosphatidylcholines is used as both a supporting biomimeticsurface and part of a signal transduction element. The pyrene excimer formed in the surface can act asfluorescence donor, and DABCY/BODIPY-FL covalently attached to receptor (GM1) can act as acceptors.Aggregation of the acceptor-tagged receptors resulting from multivalent binding of CT induces a decreasein efficiency of fluorescence quenching of the pyrene excimer by DABCY or energy transfer from pyreneexcimer to BODIPY-FL. In the case using fluorescent acceptors that can undergo distance-dependentfluorescence self-quenching, combination of the lower energy transfer efficiency from the excimer and theacceptor's self-quenching capabililty make acceptor fluorescence go down even further by the binding. Thisscheme can achieve signal amplification and high surface density of the optical transduction elements,which, in return, require relatively small surface area.