In Vitro Studies on the Interaction of Phytase with Trypsin and Amylase Extracted from Shrimp (Penaeus vannamei) Hepatopancreas

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• 作者：S. Divakaran and Anthony C. Ostrowski
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1998
• 出版时间：December 1998
• 年：1998
• 卷：46
• 期：12
• 页码：4973 - 4976
• 全文大小：41K
• 年卷期：v.46,no.12(December 1998)
• ISSN：1520-5118

文摘

Phytases are added to livestock feeds to enhance bioavailability of phytate phosphorus. The possibilityof adding phytase to shrimp feeds was investigated by studying the in vitro interaction of phytaseon trypsin and amylase extracted from the hepatopancreas (HP extract) of Penaeus vannamei.Proteolysis of soybean meal (SBM) protein measured as tryptic activity increased linearly withincreasing phytase units, indicating that phytase may exert an indirect but positive effect on SBMprotein hydrolysis. Increase in tryptic hydrolysis was not significantly affected by changes in pHfrom 5.5 to 8.5. An additive effect was observed between trypsin and possibly other proteolyticenzymes in the HP extract and phytase on the release of phosphorus from phytate in SBM. Aninhibitory effect on HP extract amylase activity by phytase, reported here for the first time, mayhave important implications on direct addition of phytase to shrimp feeds.Keywords: Phytase; shrimp; trypsin; amylase; interaction