Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

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• 作者：Yihao Zhang ; Long Qian ; Weijia Wei ; Yu Wang ; Beining Wang ; Pingping Lin ; Wenchao Liu ; Luze Xu ; Xiang Li ; Dongming Liu ; Sida Cheng ; Jiaofeng Li ; Yixuan Ye ; Hang Li ; Xiaohan Zhang ; Yiming Dong ; Xuejin Zhao ; Cuihua Liu ; Haoqian M. Zhang ; Qi Ouyang ; Chunbo Lou
• 刊名：ACS Synthetic Biology
• 出版年：2017
• 出版时间：February 17, 2017
• 年：2017
• 卷：6
• 期：2
• 页码：211-216
• 全文大小：380K
• ISSN：2161-5063

文摘

We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.