Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

详细信息    查看全文

• 作者：Weixun Wang ; Haihong Zhou ; Hua Lin ; Sushmita Roy ; Thomas A. Shaler ; Lander R. Hill ; Scott Norton ; Praveen Kumar ; Markus Anderle ; and Christopher H. Becker
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：September 15, 2003
• 年：2003
• 卷：75
• 期：18
• 页码：4818 - 4826
• 全文大小：216K
• 年卷期：v.75,no.18(September 15, 2003)
• ISSN：1520-6882

文摘

A new method is presented for quantifying proteomic andmetabolomic profile data by liquid chromatography-massspectrometry (LC-MS) with electrospray ionization. Thisbiotechnology provides differential expression measurements and enables the discovery of biological markers(biomarkers). Work presented here uses human serumbut is applicable to any fluid or tissue. The approach relieson linearity of signal versus molecular concentration andreproducibility of sample processing. There is no use ofisotopic labeling or chemically similar standard materials.Linear standard curves are reported for a variety ofcompounds introduced into human serum. As a measureof analytical reproducibility for proteome and metabolomesampling, median coefficients of variation of 25.7 and23.8%, respectively, were determined for ~3400 molecular ions (not counting their numerous isotopes) from 25independently processed human serum samples, corresponding to a total of 85 000 individual molecular ionmeasurements.