Microarray Based Screening of Peptide Nano Probes for HER2 Positive Tumor

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• 作者：Zihua Wang ; Weizhi Wang ; Xiangli Bu ; Zewen Wei ; Lingling Geng ; Yue Wu ; Chengyan Dong ; Liqiang Li ; Di Zhang ; Shu Yang ; Fan Wang ; Christopher Lausted ; Leroy Hood ; Zhiyuan Hu
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：August 18, 2015
• 年：2015
• 卷：87
• 期：16
• 页码：8367-8372
• 全文大小：401K
• ISSN：1520-6882

文摘

Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aK_D of 10^鈥? M were obtained from a 10⁵ library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.